Inicio > Àrea de Ciències i Tecnologia > Microcredencial Universitària en Good Practices in Cytometry

1a Edició - Codi 22827010

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[ARG_VENTA2] => [AÑO_CURSO_DESC] => [MODALIDAD_EVALUACION] => [MODALIDAD_EVALUACION2] => On-line activities of theoretical type (recorded presentations, videos and access to research articles and reviews) and of practical type (remote connection to cytometer simulators and to operating cytometers, Off-line study of materials and off-line work on real cytometric data files, guided management of cytometry simulators and resolution computer programs of case studies. [OBSERVACION_MATRICULA_1] => [OBSERVACION_MATRICULA_2] => [OBSERVACION_MATRICULA_3] => [SALIDA_PROFESIONAL] => Acquiring experience in Flow Cytometry for basic and clinical applications. Preparing the ESCCA European Certificate for Cytometry Operators [CRITERIO_ADMISION] => [CRITERIO_ADMISION2] => [CRITERIO_ADMISION3] => [FORMACION_APRENDIZAJE] => [FORMACION_APRENDIZAJE2] => [FORMACION_APRENDIZAJE3] => [ANO_CURSO_DESC] => Curs 2022/2023 [programa] => Array ( [0] => Array ( [CODIGO_CURSO] => 22827010 [AÑO_CURSO] => 34 [CODIGO] => 1 [NOMBRE_MATERIA] => Good Practices in Cytometry [NOMBRE_MATERIA_VAL] => Good Practices in Cytometry [DESCRIPCION] => programa || programa2 || programa3 [DESCRIPCION1] => 1. FUNDAMENTALS AND APPLICATIONS OF FLOW CYTOMETRY: 1.1 Technical basis of flow cytometry. 1.2 Overview of general applications of flow cytometry. 1.3 Fluorescence and fluorescent markers. 1.4 Components and operation of the flow cytometer: Fluidic System 1.5 Components and operation of the flow cytometer: Optical System 1.6 Components and operation of the flow cytometer: Electronic System 1.7 Generation, Presentation, Storage and Data Management in Flow Cytometry 1.8 Recent technological advances in Flow Cytometry 1.9 Cytometry resources on the Internet. 2. DESIGN AND OPTIMIZATION OF PRE-ANALYTIC EXPERIMENTS: 2.1 Essential issues for experimental design in Flow Cytometry. 2.2 Differential technical characteristics of existing flow cytometers. 2.3 Collection, Storage and Preparation of samples: Clinical cytometry. 2.4 Collection, Storage and Preparation of samples: Basic cytometry. 2.5 Considerations for the selection of reagents for Flow Cytometry 2.6 Internet tools for reagent selection for flow cytometry 2.7 Internet tools for panel design in Flow Cytometry 2.8 Rules for the design and optimization of panels in Flow Cytometry 2.9 Controls in Flow Cytometry 3. DESIGN AND OPTIMIZATION OF EXPERIMENTS-ANALYTICAL PHASE: 3.1 Connecting and disconnecting the cytometer. 3.2 Cleaning and maintenance of the cytometer. 3.3 Standardization and quality control. 3.4 Acquisition of data with the cytometer's software. 3.5 Strategies for population selection (¿Gating¿). 3.6 Fluorescence compensation. 3.7 Cell separation by flow cytometry ("Cell Sorting") 3.8 Occupational Safety Risks and Procedures in Flow Cytometry. 3.9 Detection of common problems and troubleshooting. 4. ANALYSIS, INTERPRETATION AND DATA MANAGEMENT. 4.1 Specific format of final data in flow cytometry (FCS format). 4.2 Conventional statistical methods in flow cytometry. 4.3 Advanced statistical and bioinformatics methods in flow cytometry. 4.4 Specific statistics for the flow cytometry of rare events. [DESCRIPCION2] => 4.5 Modeling methods for cell cycle analysis and proliferation by flow cytometry. 4.6 Ratiometric methods in flow cytometry. 4.7 Quantitative flow cytometry 4.8 Fusion of files in flow cytometry and virtual immunophenotype. 4.9 Introduction to cytometer- interfaced software, third-party softwareand public domain software. 5. PRACTICAL EXERCISES: RESOLUTION OF REAL CASES 5.1 Guided practical exercises of panel design in Flow Cytometry. 5.2 Instructions and recommendations for the use of cytometers own software, commercial licensed software and public domain software. 5.3 Exercises for analysis of real cases optimized for resolution with cytometers own software, commercial software with use license or public domain software. [DESCRIPCION3] => [DESCRIPCION1_VAL] => 1. FUNDAMENTALS AND APPLICATIONS OF FLOW CYTOMETRY: 1.1 Technical basis of flow cytometry. 1.2 Overview of general applications of flow cytometry. 1.3 Fluorescence and fluorescent markers. 1.4 Components and operation of the flow cytometer: Fluidic System 1.5 Components and operation of the flow cytometer: Optical System 1.6 Components and operation of the flow cytometer: Electronic System 1.7 Generation, Presentation, Storage and Data Management in Flow Cytometry 1.8 Recent technological advances in Flow Cytometry 1.9 Cytometry resources on the Internet. 2. DESIGN AND OPTIMIZATION OF PRE-ANALYTIC EXPERIMENTS: 2.1 Essential issues for experimental design in Flow Cytometry. 2.2 Differential technical characteristics of existing flow cytometers. 2.3 Collection, Storage and Preparation of samples: Clinical cytometry. 2.4 Collection, Storage and Preparation of samples: Basic cytometry. 2.5 Considerations for the selection of reagents for Flow Cytometry 2.6 Internet tools for reagent selection for flow cytometry 2.7 Internet tools for panel design in Flow Cytometry 2.8 Rules for the design and optimization of panels in Flow Cytometry 2.9 Controls in Flow Cytometry 3. DESIGN AND OPTIMIZATION OF EXPERIMENTS-ANALYTICAL PHASE: 3.1 Connecting and disconnecting the cytometer. 3.2 Cleaning and maintenance of the cytometer. 3.3 Standardization and quality control. 3.4 Acquisition of data with the cytometer's software. 3.5 Strategies for population selection (¿Gating¿). 3.6 Fluorescence compensation. 3.7 Cell separation by flow cytometry ("Cell Sorting") 3.8 Occupational Safety Risks and Procedures in Flow Cytometry. 3.9 Detection of common problems and troubleshooting. 4. ANALYSIS, INTERPRETATION AND DATA MANAGEMENT. 4.1 Specific format of final data in flow cytometry (FCS format). 4.2 Conventional statistical methods in flow cytometry. 4.3 Advanced statistical and bioinformatics methods in flow cytometry. 4.4 Specific statistics for the flow cytometry of rare events. [DESCRIPCION2_VAL] => 4.5 Modeling methods for cell cycle analysis and proliferation by flow cytometry. 4.6 Ratiometric methods in flow cytometry. 4.7 Quantitative flow cytometry 4.8 Fusion of files in flow cytometry and virtual immunophenotype. 4.9 Introduction to cytometer- interfaced software, third-party softwareand public domain software. 5. PRACTICAL EXERCISES: RESOLUTION OF REAL CASES 5.1 Guided practical exercises of panel design in Flow Cytometry. 5.2 Instructions and recommendations for the use of cytometers own software, commercial licensed software and public domain software. 5.3 Exercises for analysis of real cases optimized for resolution with cytometers own software, commercial software with use license or public domain software. 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Universitat de València [CARGO_FACULTAD] => Catedrático/a de Universidad [NPI] => G9280 [EMAIL_FACULTAD] => Jose.E.Oconnor@uv.es [CARGO_EMPRESA] => [DIRECCION_URL_POSTGRADO] => [URL_LINKEDIN_POSTGRADO] => ) [4] => Array ( [DNI] => emp370777 [NOMBRE_PERSONA] => Jordi [APELLIDOS] => Pétriz González [PDI] => 4 [DEPARTAMENTO_FACULTAD] => [CARGO_FACULTAD] => [NPI] => [EMAIL_FACULTAD] => [CARGO_EMPRESA] => Jefe de Laboratorio, Grupo de Citómica Funcional - Institut d'investigació contra la Leucèmia Josep Carreras [DIRECCION_URL_POSTGRADO] => [URL_LINKEDIN_POSTGRADO] => ) ) [direccio] => Array ( [0] => Array ( [0] => Array ( [DNI] => uni29506 [NOMBRE_PERSONA] => José Enrique [APELLIDOS] => O'Connor Blasco [PDI] => 1 [DEPARTAMENTO_FACULTAD] => Departament de Bioquímica i Biologia Molecular. Universitat de València [CARGO_FACULTAD] => Catedrático/a de Universidad [NPI] => G9280 [EMAIL_FACULTAD] => Jose.E.Oconnor@uv.es [CARGO_EMPRESA] => [DIRECCION_URL_POSTGRADO] => [URL_LINKEDIN_POSTGRADO] => ) ) ) )

Microcredencial Universitària en Good Practices in Cytometry

Dades generals
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 Programa
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 Direcció
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 Professorat
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 Metodologia
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 Accés i Resultats d'Aprenentatge
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Dades generals

Curs acadèmic: Curs 2022/2023

Tipus de curs: Microcredencial Universitari

Nombre de crèdits: 3.00 Crèdits ECTS

Preinscripció al curs: Hasta el 05/11/2022

Data inici: Novembre 2022

Data fi: Desembre 2022

Matrícula: 350 € (import preu públic)

Modalitat: On-line

Lloc d'impartició: Online

Horari: Monday-Friday 18.00-20.00 hours

Més informació

Objectius del curs

The general objective of the course is to provide students with specialized knowledge in flow cytometry that will include the correct handling of the instruments, the design and application of cytometric analysis procedures, the acquisition and management of data and the interpretation of results.

Objectius professionals

Acquiring experience in Flow Cytometry for basic and clinical applications.

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